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Nucleic acid based HIV and SIV therapies

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CDA049 Abstract Spreading epidemic of protease codon 35 inserted strains from HIV-1 subtype C in Portugal
V. Duque1, J. Pereira-Vaz1, C. Valente2, C. Morais1, V. Mota1, J.G. Saraiva-da-Cunha3, A. Meliço-Silvestre3
1Hospitais da Universidade de Coimbra, Laboratório de Virologia do Departamento de Doenças Infecciosas, Coimbra, Portugal, 2Centro Hospitalar de Coimbra, Serviço de Doenças Infecciosas, Coimbra, Portugal, 3Hospitais da Universidade de Coimbra, Departamento de Doenças Infecciosas, Coimbra, Portugal

CDA050 Abstract
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Near full-length genome characterization of HIV-1 intrasubtype B clusters and recombinants in Panama
S.M. Ahumada-Ruiz1,2, D. Flores-Figueroa3, I. Toala-González3, M.M. Thomson1
1Instituto de Salud Carlos III, Madrid, Spain, Departamento de Patogenia Viral, Centro Nacional de Microbiología, Madrid, Spain, 2Universidad de Panamá, Laboratorio de Microbiología Experimental y Aplicada, Panamá, Panama, 3Caja del Seguro Social de Panamá, Departamento de Enfermedades Infecciosas, Panamá, Panama

CDA051 Abstract Prevalence and resistance mutations of non-B HIV subtypes among immigrants in Southern Spain
B. de Felipe1, P. Viciana1, L.F. Lopez-Cortes1, F. Fernandez-Cuerda2, M. Trastoy1, R. Mata1, M. Leal1, A. Vallejo1
1Hospital Virgen del Rocio, Infectious Diseases Service, Seville, Spain, 2Hospital Virgen Macarena, Microbiology Service, Seville, Spain

CDA052 Abstract
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Characterization of HIV-1 CRF01_AE integrase gene in a cohort of Vietnamese drug users
R. Barbara1, C. Wanke2, H. Nguyen3, L. Trinh3, D. Dang3, M. Jordan4
1Tufts University, Geographic Medicine and Infectious Disease, Boston, United States, 2Tufts University, Boston, United States, 3National Institute of Infectious and Tropical Diseases, Hanoi, Viet Nam, 4Tufts University School of Medicine, Infectious Disease and Geographic Medicine, Boston, United States

CDA053 Abstract High HIV-1 genetic complexity in men who have sex with men (MSM) in Bangkok, Thailand
W. Leelawiwat1, M. Arroyo2, F. Mueanpai1, O. Kongpechsatit1, C. Kittinunvorakoon1, V. Assawadarachai2, M. de Souza2, S. Chaikummao1, W. Chonwattana1, J. Tongtoyai1, A. Sangiamkittikul1, F. van Griensven1,3, J. Mcnicholl1,4
1Thailand MOPH - U.S. CDC Collaboration, Nonthaburi, Thailand, 2The Armed Forces Research Institute of Medical Sciences, Department of Retrovirology, Bangkok, Thailand, 3Centers for Disease Control and Prevention, Atlanta, GA, United States, 4Centers for Disease Control and Prevention, Atlanta, United States

CDA054 Abstract Impact of treatment with reverse transcriptase inhibitors on the variation of protease region among HIV-1 infected individuals
S. Uma1, S. Suniti1, K.G. Murugavel1, N. Kumarasamy1, S.S. Sunil1, H.M. Kenneth2, P. Balakrishnan1
1Y.R.Gaitonde Centre for AIDS Research and Education, Chennai, India, 2Brown University/Miriam Hospital, Providence, United States

CDA055 Abstract Analysis of the mean increase in CD4 cell counts and viral load decrease between different subtypes C, CRF31_BC, B and F after initial antiretroviral therapy in a Porto Alegre HIV clinic, southern of Brazil
C. Nunes1, C. Dias1, I. Lamego1, L. Brigido2
1Porto Alegre City Bureau of Healthy, HIV Clinic, Porto Alegre, Brazil, 2Adolfo Lutz, Virology Laboratory, Sao Paulo, Brazil

CDA056 Abstract High frequency of SI/X4 phenotype and ex vivo replicative capacity in CRF14_BG primary isolates compared with their parental subtypes B and G
M. Muñoz-Nieto1, M.T. Cuevas2, A. Fernández-Garcia2, M. Pinilla2, A. Ocampo3, V. García2, E. Delgado2, A.M. Sánchez2, M. Thomson2, L. Pérez-Álvarez2
1Centro Nacional de Microbiología. Instituto de Salud Carlos III, Madrid, Spain, 2Patogenia viral. Centro Nacional de Microbiología. Instituto de Salud Carlos III, Madrid, Spain, 3Complejo Hospitalario Xeral-Cíes., Vigo, Pontevedra, Spain

CDA057 Abstract Quantification of plasma HIV-RNA levels in non-B subtypes and URFs with three commercial assays
B. Bruzzone1, P. Caligiuri2, F. Bisio3, N. Nigro1, A. Ventura2, F. Mayinda Mboungou4, L. Miguel4, A. Nzagou4, E. Malfatti5, C. Viscoli3, G. Icardi2
1San Martino Hospital, Hygiene Unit, Genoa, Italy, 2University of Genoa, Health Sciences, Genoa, Italy, 3University and San Martino Hospital, Infectious Disease Unit, Genoa, Italy, 4Kento-Muana Laboratory, Pointe Noire, Congo, 5Eni Foundation, Rome, Italy

CDA058 Abstract Development of real time PCR targeting protease to discriminate HIV-1 from subtypes B and F
D. Teixeira, P. Munerato, S.C.V. Komninakis, E.E. Fusuma, M.C.A. Sucupira, R.S. Diaz
Federal University of São Paulo, Medicine Department, São Paulo, Brazil

CDA059 Abstract
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Genetic characterization of HIV-1 among antiretroviral-naïve persons in rural Western Kenya
L. Nafisa1, S. Inzaule1, A. Kasembeli1, H. Musuluma1, B. Oyaro1, J. Okonji1, T. Thomas2, H. Vandenhoudt3, P. Amornkul4, K. Laserson2, C. Zeh2
1Kenya Medical Research Institute (KEMRI), HIV-Research, Kisumu, Kenya, 2US Centers for Disease Control and Prevention, Lab, Kisumu, Kenya, 3KEMRI/Institute of Tropical Medicine, Lab, Kisumu, Kenya, 4International AIDS Vaccine Initiative USA, Lab, California, United States

CDA060 Abstract
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Genetic diversity and antiretroviral drug resistance mutations in HIV-1 infected patients from Cape Verde Islands
V. Oliveira1, I. Bártolo2, E. Valadas3, J. Barreto4, E. Almeida4, F. Antunes3, N. Taveira5,6
1IPO, Lisboa, Portugal, 2Faculdade de Farmácia de Lisboa, URIA-CPM, Lisboa, Portugal, 3Clínica Universitária de Doenças Infecciosas/Serviço de Doenças Infecciosas, Faculdade de Medicina de Lisboa/Hospital de Santa Maria, Lisboa, Portugal, 4Hospital Dr. Agostinho Neto, Cidade da Praia, Cape Verde, 5Instituto Superior de Ciências da Saúde Egas Moniz, Almada, Portugal, 6Faculdade de Farmácia de Lisboa, URIA-CPM, Microbiologia, Lisboa, Portugal


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